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We isolated two insertions that affected expression of DtxR, one 121 bp upstream of dtxR and the other within an essential region of the dtxR coding sequence, ...
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Construction and purification of a His-tagged DtxR protein. A PCR product fusing the coding region of the C. glutamicum dtxR gene with a nucleotide sequence ...
Jan 15, 2005 · ABSTRACT This report describes a genetic locus associated with siderophore biosynthesis and transport in Corynebacterium diphtheriae.
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Experimental Procedures. Construction of Mutant dtxR Genes. The QuikChange mutagenesis protocol (Stratagene) was used to mutate the dtxR gene in pROM-dtxR, pROM ...
Apr 15, 2006 · The construction of the plasmids used as templates in the transcription reaction is described below. To construct pBSdtxR1, the 1,400-bp PCR ...
Construction of dtxR mutations by random mutagenesis with sodium bisulfite ... Construction of dtxR mutations by oligonucleotide-directed site-specific.
Jan 31, 2024 · We isolated two insertions that affected expression of DtxR, one 121 bp upstream of dtxR and the other within an essential region of the dtxR ...