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What does the protein DtxR do?
What does dimerized DtxR bind to?
We isolated two insertions that affected expression of DtxR, one 121 bp upstream of dtxR and the other within an essential region of the dtxR coding sequence, ...
Construction and purification of a His-tagged DtxR protein. A PCR product fusing the coding region of the C. glutamicum dtxR gene with a nucleotide sequence ...
diphtheriae, a DtxR repressor titration assay (DRTA) was developed and used to identify 10 previously unknown DtxR binding sites. Open reading frames downstream ...
We demonstrate that the ancillary metal-binding site mutant DtxR(H79A) requires higher concentrations of metal ions for activation both in vivo and in vitro, ...
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Apr 15, 2006 · The construction of the plasmids used as templates in the transcription reaction is described below. To construct pBSdtxR1, the 1,400-bp PCR ...
Construction of dtxR mutations by random mutagenesis with sodium bisulfite ... Construction of dtxR mutations by oligonucleotide-directed site-specific.
... (DtxR), the low level of reporter gene expression has limited the detection and analysis of mutations affecting subtle changes in repressor–operator binding.